Chapter 3.10

DNA Sequence Analysis Onjectives and Resources

This presentation describes pyrosequencing, restriction fragment length polymorphism, chain terminators, ASO, manual sequence analysis, and automated sequence analysis methods to sequence DNA. (29 slides)

Objectives

Describe the principles and uses for the following:

  • Pyrosequencing
  • Restriction Fragment Length Polymorphism (RFLP)
  • Chain Terminators (Sanger Method)
  • Allele Specific Oligonucleotide (ASO)
  • Manual Sequence Analysis
  • Automated Sequence Analysis 

http://www.dnalc.org/resources/3d/index.html - Sanger Sequencing 

http://www.dnalc.org/resources/3d/index.html In Dolan DNA Learning Center, see Flyover – a close look at chromosome 11, and Chromosome Close Up which contains a 3-D animation of chromosome coiling from DNA to nucleosome to mitosis.  It also includes information on X chromosome banding areas, a 2-D animation including explanations of exons and introns.  Genome mining includes information about transcription, 3-D animation of promotors, TATA boxes, etc.  Genome FISHing has a video regarding variability in the genome such as SNPs and RLFPs.  Genome Spots compares various areas of chromosomes as to their variability, genes encoded, etc.

http://www.dnalc.org/resources/3d/index.htmlSorting and Sequencing – Gel 2-D, Early DNA Sequencing 2-D, Cycle Sequencing 2-D