Chapter 3.3

Nucleic Acid Extraction Methods Objectives and Resources

This presentation discusses the types of specimens that may be used to isolate DNA, various methods for extraction of both DNA and RNA, methods to determine the quantity and quality of nucleic acid preparations, methods to calculate the concentration and yield of DNA and RNA from a given preparation, and trouble shooting techniques to address common DNA extraction problems.

(32 slides)

Objectives:

  • List the types of specimens that are acceptable for isolating nucleic acids
  • Compare and contrast organic, inorganic, and solid phase approaches for DNA isolation.
  • Compare and contrast organic and solid phase approaches for isolating total RNA. 
  • Describe the gel-based, spectrophotometric fluorometric methods used to determine the quantity and quality of DNA and RNA preparations.
  • Calculate the concentration and yield of DNA and RNA from a given nucleic acid preparation.
  • Identify trouble-shooting techniques used to address common problems involving DNA extraction 

Actual Lab:  Website:  http://learn.genetics.utah.edu/content/labs/extraction/howto/  The extraction of DNA from anything living.  A kitchen level actual lab, which shows the principles of DNA extraction.

Virtual Lab:  http://learn.genetics.utah.edu/  DNA Extraction

Readings:  MD, Chapter 4