Chapter 3.4

Separation and Detection Part I Objectives and Resources

This presentation discusses electrophoresis, nucleic acid hybridization, and Southern, Northern, and Western blotting. (39 slides)

Objectives:

  • Discuss the principles of electrophoresis as it applies to nucleic acids.
  • List the similarities and differences between agarose and polyacrylamide gels and uses of each.
  • Explain the use of buffers and additives in electrophoresis.
  • Discuss capillary electrophoresis.
  • Describe the types of equipment used for electrophoresis and how samples are handled for the procedure.
  • Discuss differences and similarities between pulse field gel electrophoresis and regular electrophoresis regarding method and applications.
  • Discuss detection systems used in nucleic acid applications.

http://www.dnalc.org/ddnalc/resources/electropheresis.html – gel electropheresis

http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120078/bio_g.swf::Southern%20Blot – Southern blot

http://www.gene-quantification.de/movoie.html  Northern blot

Virtual Lab:  Gel Electrophoresis – http://learn.genetics.utah.edu/

Actual Labs:  Colorful Electrophoresis, Build a Gel Electrophoresis Chamber.

http://teach.genetics,utah,edu/content/begin/dna/electrophoresis/index.html

Resource:  Roche CD, Molecular Technology: Microarrays, in situ Hybridization, Agarose Gel Electrophoreses, SDS-PAGE, Southern, Northern, and Western Blots, Troubleshooting

Readings:  MD, Chapter 5 and Chapter 6: 94-102