Chapter 3.4

Separation and Detection Part I Objectives and Resources

This presentation discusses electrophoresis, nucleic acid hybridization, and Southern, Northern, and Western blotting. (39 slides)


  • Discuss the principles of electrophoresis as it applies to nucleic acids.
  • List the similarities and differences between agarose and polyacrylamide gels and uses of each.
  • Explain the use of buffers and additives in electrophoresis.
  • Discuss capillary electrophoresis.
  • Describe the types of equipment used for electrophoresis and how samples are handled for the procedure.
  • Discuss differences and similarities between pulse field gel electrophoresis and regular electrophoresis regarding method and applications.
  • Discuss detection systems used in nucleic acid applications. – gel electropheresis – Southern blot  Northern blot

Virtual Lab:  Gel Electrophoresis –

Actual Labs:  Colorful Electrophoresis, Build a Gel Electrophoresis Chamber.


Resource:  Roche CD, Molecular Technology: Microarrays, in situ Hybridization, Agarose Gel Electrophoreses, SDS-PAGE, Southern, Northern, and Western Blots, Troubleshooting

Readings:  MD, Chapter 5 and Chapter 6: 94-102