Chapter 3.6

FISH Objectives and Resources

This presentation discusses in more detail the principles of in situ hybridization and its use in FISH and CISH. (16 slides)

Objectives: 

  • Define FISH and its purpose for use in the clinical laboratory
  • Distinguish between FISH and CISH
  • Explain the basic steps in an ISH procedure
  • Identify the cause of common problems in the procedure
  • Describe amplification techniques used to detect small targets

Readings:  MD, Chapter 6:  103 – 120