Chapter 3.7

Nucleic Acid Amplification Objectives and Resources

This presentation discusses the principles and basic steps of PCR.  The advantages and disadvantages of PCR are discussed, as well as the importance of contamination control when using these techniques.  Applications which utilize PCR are also discussed.  (58 slides)

Objectives:

  • Describe the principle and basic steps of amplification by polymerase chain reaction (PCR)
  • List the advantages and limitations of PCR
  • Describe the importance of contamination control in labs performing PCR
  • List PCR applications

http://www.cytologystuff.com/indexmolecular.htm

Molecular Methods – Amplification

1.     PCR

Virtual Lab – Website:  http://learn.genetics.utah.edu/

Click on “Virtual Labs” and then “PCR”

Resources:

Roche CD, 2001, Polymerase Chain Reaction (This CD may no longer be available.) 

http://www.roche.com/home/science/sci_gengen_cdrom.htm

(Click on order form)

  • PCSEC1.ppt – DNA to PCR (30 slides)
  • PCSEC2.ppt – In the test tube (12 slides)
  • PCSEC3.ppt – Minimal requirements (2 slides)
  • PCSEC4.ppt – Machines used in the process (31 slides) 
  • (PCSEC1 and 2 above are best to learn PCR basics.)
  • Glossary – List of important terms.

www.dnalc.org/ddnalc/resources/pcr.html  - animation of PCR cycles

http://www.dnalc.org/resources/3d/index.html Dolan DNA Learning Center – DNA Interactive – Code. Manipulation, Techniques:  

Amplifying – Making many copies 2-D and Amplifying – PCR 3-D

Readings:  MD, Chapter 7, 121-143