Chapter 3.8

Alternatives to PCR – I Objectives and Resources

This presentation discusses amplification techniques other than PCR such as TAS, TMA, NASBA, RCA, LCR, SDA, and Qβ.   (20 slides)


  • Discuss the principles and specific components involved with the various techniques for the amplification of target nuclei acids, probes or signals
Molecular Methods – Amplification

  • Invader
  • Hybrid Capture
  • TMA
  • SDA
  • LCR 

Readings:  MD, Chapter 7, 144-149