Chapter 6.1

Fluorescence in Situ Hybridization (FISH)-Principles and Preparatory Techniques Objectives and Resources

This section describes the use of FISH in the detection of neoplasia.  The use of FISH for the analysis of urothelial carcinoma, the development and validation of UroVysion testing, and how to prepare the specimen for testing are also discussed.  Probes, both centromeric and locus specific, are discussed, as well as other applications in conditions such as biliary tract cancer, Barrett’s esophagus, cervical cancer, and lung cancer.  The usefulness of cytologic urine specimens vs. other methods of detection are compared to the FISH method of screening for the presence or absence of certain molecular targets. Methodologies to prepare the slides for FISH are examined.  (51 slides)